Imaging Facility

Jump to: 

• Zeiss LSM 800
• Zeiss LSM 880
• Quorum Technologies X-Light V2 Spinning Disc 

 

Zeiss LSM 800 

The Zeiss LSM 800 is a laser scanning microscope system equipped with an inverted fluorescence microscope, four laser lines, highly sensitive GaAsP detectors, an Airyscan detector, and a transmitted light detector. It is capable of simultaneously acquiring 4 fluorescent images and 1 DIC image in a single sample scan and is able to do three-dimensional fluorescence imaging. Technical specifications are as follows. 

Microscope 

• Observer Z1 

• High-end fully motorized inverted microscope 

• Remote LCD touch screen allowing for remote control of all motorized components 

• Motorized Z motor with 10nm reproducibility and customizable variable speed focus control 

• Motorized XY scanning stage, minimum travel range of 130x85mm, with <1µm reproducibility.  Capable of holding slides, Lab-Tek chambers, 35mm dishes. 

• Motorized light path control, condenser, illumination shutters, reflector turret, filter cubes 

• Hardware-based focus stabilization, capable of being used for multiple xy locations in combination with z-stack, time course, and spectral imaging, in order to optimize acquisition from live cell experiments 

Confocal 

• XY image resolution of 6000x6000 pixels 

• Spectral detection with a spectral resolution as low as 1nm, with a range of 400-720nm (detection available >720) 

• Spectral un-mixing of up to 10 fluorophores, with 20 spectral channels 

• 360-degree rotation of scan field 

• Multi-Region of Interest FRAP capability, with ability to vary laser power between FRAP locations 

Objectives 

• EC Plan-Neofluar 10x/0.5  

• Plan Apochromat 20x/0.8 NA 

• Plan Apochromat 40x/1.4 NA oil immersion 

• Plan Apochromat 63x/1.4NA oil immersion  

• Differential Interference Contrast for all objectives 

Lasers 

Full complement of solid-state lasers, with the following wavelengths and minimum powers, or equivalent. Laser line intensity is variable from 1-100% in 0.1% increments, with full linear response. 

• 405nm (5mw) 

• 488nm (10mw) 

• 555nm (10mw) 

• 639mw (5mw) 

Detectors 

• Capable of simultaneously acquiring 4 fluorescent images and 1 DIC image in a single sample scan 

• Two internal high-sensitivity low noise Gallium Arsenide Phosphide detectors (GaAsP) 

• One high-sensitivity low noise super resolution detector (Airyscan) 

• Single transmitted light detector for DIC imaging in parallel with confocal 

Widefield Fluorescence 

• Filters for DAPI, GFP, and Cy3, or equivalent dyes 

• Alignment-free extended long life (20000hr) LED fluorescence light source 

Table 

• Actively dampened isolation table 

Computer 

• Windows 7 professional 

• Confocal Operating Software Zen 2.3 

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Zeiss LSM 880 

The Zeiss LSM 880 is a laser scanning microscope system equipped with an upright fluorescence microscope, four conventional laser lines, and a tunable Insight X3 laser for 2-photon imaging. It has GaAsP detectors, an Airyscan detector, and Big-2 detector for 2-photon imaging. The system delivers super-resolution imaging and fast scanning speed, and is able to do three dimensional imaging. The 2-photo imaging mode, with a long working distance objective, is capable of acquiring optical sections of deep tissue layers. Technical specifications are as follows: 

Microscope   

• Examiner Z1, upright 

• Objectives are changed manually 

• Z-Piezo stage 

Objectives 

• EC Plan 10X /0.3 DIC, dry 

• W Plan Apo 20x/ 1 w DIC-III, water 

• LD LCI Plan-Apo 25x/0.8 W DICII, water / glycerol with or without cover slip / oil with cover slip 

• Plan-Apo 63x/1.4 Oil DIC II, with cover slip 

Lasers 

• Diode 405nm 

• Argon 458nm, 488nm, 514nm 

• DPSS 561nm 

• HeNe 633 

Detectors 

• Gallium Arsenide Phosphide detectors (GaAsP) 

• One high-sensitivity low-noise super-resolution detector (Airyscan) 

• BiG-2 GaAsP detector for 2-photon imaging 

2-Photon 

• InsightX3 Tunable laser; 690-1080 nm 

• Emission filters for green (500-550 nm) and red (575-610 nm)  

• BIG-2 GaAsP detector 

Wild field Fluorescence 

Filter sets available: 

• DAPI, Zeiss filter set 49 

• Alexa 488, Zeiss filter set 38 

• Alexa 555, Zeiss Filter set 43 

Light source: 

• X-cite 110 LED 

Table 

• Newport ST Series 

Computer 

• Windows 7 

• Operating Software Zen 2.3 SP1 

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Quorum Technologies X-Light V2 Spinning Disc 

This acquisition system is an X-Light V2 spinning disk confocal with an LDI-7 laser engine, supplied and integrated by Quorum Technologies. The system is equipped with a stage-top live cell incubator for live cell imaging in 35 mm dishes, or 12-, 24-, 48-, or 96-well plates. Imaging with a confocal spinning disk is useful for high-speed imaging of living cells expressing fluorescent proteins or stained with membrane-permeable synthetic dyes. Photo-bleaching and photo-toxicity are reduced with spinning disk microscopy. Technical specifications are as follows: 

Microscope   

• Leica DMi8 inverted microscope with an ASI motorized stage 

Objectives 

• 10x, dry 

• 20x, dry, long working distance 

• 40x, dry 

• 63x, water 

• 63x, oil 

Lasers 

Excitation wavelengths: 

• 405 nm 

• 445 nm 

• 470 nm 

• 520 nm 

• 528 nm 

• 555 nm 

• 640 nm 

Emission filters: 

• 460/50m 

• 525/50m 

• 610/75m 

• 700/75m 

• 528/640m (dual Green Red) 

• 445/520m (dual CFP YFP) 

Camera 

Prime HS:  sCMOS camera 

Widefield Fluorescence   

Filter sets available: 

• DAPI; 11525304; Excitation: 350/50; Emission 460/50; Dichroic: 400 

• Y5, size P; 11525312; Excitation: 620/60; Dichroic: 660; Emission: 700/75 

• FITC; 11525307; Excitation: 480/40; Dichroic: 505; Emission: 527/30 

• RHOD; 11525308; Excitation: 546/10; Dichroic: 560; Emission: 585/40 

Lamp: 

• X-Cite 110 LED, Lumen Dynamics 

Live Cell incubation   

• Chamlide TC-W stage-top incubator 

• FC-5N CO2/Air Mixer 

Computer 

• Dell 7820 running Windows 10 

• Acquisition software Metamorph v7.10.5 

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Condenser 

• Phase contrast, NA 0.9, dry 

Objectives 

• Nikon Plan UW 1x/0.04 na 

• Nikon Plan Fluo 4x /0.13 na 

• Nikon Plan Fluo 10x/0.25 na 

• Nikon Plan Fluo 20x/0.40 na 

• Nikon Plan Fluo 100x1.30 na Oil 

Fluorescence Filters 

• ET DAPI 

• ET GFP 

• ET RsRed 

• UV-2A 

Lamp 

• X-Cite 120 

Imaging Software 

• NIS-Elements BR3.0 

Camera 

• Nikon DS-Ri1 

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JEOL NeoScope JCM-5000 

The NeoScope is a benchtop scanning electron microscope (SEM) that can rapidly acquire images with high magnification, high resolution, and a large depth of field. No special sample preparation (eg. coating) is required. 

• 10 - 20,000 X magnification 

• Easy automatic or manual control of focus, contrast, and brightness 

• High and low vacuum modes 

• Three selectable accelerating voltages 

• No special sample preparation, for conductive and non-conductive samples 

Stereo microscope 

A stereo microscope for sample preparation, mounting, or dissection.  

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Imaging Facility researchers can visit the Imaging Analysis Workstation to access both commercial and open-source software that will help you take your imaging data from the microscope to publication. Some software licenses also have remote options, allowing researchers to use them from their own workstation.  

Licensed software: 

• Imaris 10.1 

• Volocity  

• Adobe Creative Cloud 

• GraphPad Prism