R. Scott Prosser
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Phone:
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Mailing Address:
3359 Mississauga Road
Mississauga ON L5L1C6
Canada
Our research focuses on dynamics and mechanism of complex biological processes such as: 1) protein folding, 2) protein misfolding and amyloidosis, 3) enzyme functional dynamics, 4) GPCR functional dynamics, and 5) Direct molecular imaging of proteins and nanoparticles by MRI. In many cases, we make use of 19F or 13C,1H NMR to detect subtle low-lying states within an ensemble, and piece together a mechanistic description. 19F NMR chemical shifts are exquisitely sensitive to molecular environments. Thus, subtle differences in conformation, folding intermediates, or different aggregation states are more likely to be resolved by 19F NMR than by other methods.
Receptors and amyloid systems represent a tremendous challenge in part because of the need for high yield expression expression of functional protein with appropriate post translational modifications. We have focused in recent years on perfecting expression in yeast which has created new opportunities for the study of conformational equilibria, dynamics, activation mechanisms, and functional pathways of complex proteins.
Courses Taught:
CHM372H, JBC472H and JCP422H (undergraduate); CHM1455F and BTC1710 (graduate)